The neighborhood is roughly bounded by Columbus Avenue and the Boston neighborhood of Roxbury to the east, Longwood Avenue to the northeast and the Olmsted designed Riverway/Jamaicaway and the town of Brookline to the west. It is northernly adjacent to the Boston neighborhood Jamaica Plain. It is served by the MBTA Green Line E Branch and the Orange Line and is within walking distance of the Museum of Fine Arts. "The Hill" overlaps with about half of the Longwood Medical and Academic Area, home to 21 world-class health care, research, education institutions and are responsible for the largest employment area in the City of Boston outside of downtown. Due to these adjacencies, the neighborhood is often struggling with institutional growth taking residential buildings and occupying storefront commercial space. But recent years have seen new retail stores, restaurants and residential development giving the neighborhood a stronger political voice and identity.
Always considered a part of Roxbury until a generation ago, Mission Hill is now most often regarded as a separate section of the city. However, neighborhood boundaries in Boston are inherently ambiguous and the subject of whether or not Mission Hill is adjacent to Roxbury or a remains a section thereof is still a subject of debate.
Mission Hill is an architectural landmark district with a combination of freestanding houses built by early wealthy landowners, blocks of traditional brick rowhouses, and many Triple decker. Many are condominiums, but there are also several two-family and some single-family homes.